wright–giemsa stain modified solution  (Millipore)

Journal: BMC Research Notes

Article Title: Cardiac regeneration in the axolotl is unaffected by alterations in leukocyte numbers induced by lipopolysaccharide and prednisolone

doi: 10.1186/s13104-021-05574-z

Figure Lengend Snippet: Systemic and local inflammatory response to pro- and anti-inflammatory stimulation. a Graphical overview of experiment. b Leukocyte numbers expressed as cell count per 5,000 red blood cell (RBC) on Wright–Giemsa stained blood smears. Significant differences shown by asterisk (grey indicating significant difference to control, red to LPS and blue to prednisolone). Representative images of axolotl leukocytes are displayed below each cell category. Error bars represent 95% confidence interval. Two-tailed unpaired students t-test with α = 0.05 was used to determine statistical significance. c Representative echocardiography images of control (left) and LPS (right) treated animals with clear pericardial fluid in control and pericardial fluid with nucleated cell infiltrate in LPS (area marked with red line). d Representative image of Wright-Giemsa stained smear of pericardial aspirate from LPS treated animal

Article Snippet: Fixated aspirate and blood smears were stained in modified Wright-Giemsa staining solution (Sigma Aldrich, cat. nr: WG128) for 45 s, rinsed in deionized water and mounted with DPX mountant (Sigma Aldrich, cat. nr: 06522).

Techniques: Cell Counting, Staining, Two Tailed Test